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Image Search Results
Journal: Immunity, Inflammation and Disease
Article Title: Bone marrow stromal cell‐derived exosomes improve oxidative stress and pyroptosis in doxorubicin‐induced myocardial injury in vitro by regulating the transcription of GSDMD through the PI3K‐AKT‐Foxo1 pathway
doi: 10.1002/iid3.810
Figure Lengend Snippet: The extraction and identification of BMSCs. (A) Ordinary optical microscope observations of BMSC morphology (×100). (B) Ordinary optical microscope analyses of adipogenic and osteogenic differentiation in BMSCs in vitro (×200). (C) Flow cytometer measurement of surface antigens (positive: CD29, CD90, and CD44; negative: CD45 and CD34). (D) Transmission electron microscope observations of BMSC‐Exos. (E) NTA analyses of BMSC‐Exo size. (F) Western blot examination of the expression of Exo surface marker proteins Alix, CD63, GM130, cytochrome c , and calnexin. (G) Laser scanning microscope evaluation of Exo uptake in H9C2 cells. BMSC, bone marrow stromal cells; Exo, exosome; NTA, nanoparticle tracking analysis.
Article Snippet: The membranes were supplemented respectively with diluted primary antibodies against Alix (ab275377, 1:1000; Abcam), CD63 (PA5‐92370, 1:1000; Thermo Fisher Scientific), GM130 (PA5‐95727, 1:2000; Thermo Fisher Scientific), cytochrome c (ab133504, 1:1000; Abcam),
Techniques: Microscopy, In Vitro, Flow Cytometry, Transmission Assay, Western Blot, Expressing, Marker, Laser-Scanning Microscopy