advance integrated scanning tool smart spm 1000 Search Results


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Hitachi Ltd advanced scanning electron microscope flexsem 1000
Advanced Scanning Electron Microscope Flexsem 1000, supplied by Hitachi Ltd, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The extraction and identification of BMSCs. (A) Ordinary optical microscope observations of BMSC morphology (×100). (B) Ordinary optical microscope analyses of adipogenic and osteogenic differentiation in BMSCs in vitro (×200). (C) Flow cytometer measurement of surface antigens (positive: CD29, CD90, and CD44; negative: CD45 and CD34). (D) Transmission electron microscope observations of BMSC‐Exos. (E) NTA analyses of BMSC‐Exo size. (F) Western blot examination of the expression of Exo surface marker proteins Alix, <t>CD63,</t> <t>GM130,</t> cytochrome c , and <t>calnexin.</t> (G) Laser scanning microscope evaluation of Exo uptake in H9C2 cells. BMSC, bone marrow stromal cells; Exo, exosome; NTA, nanoparticle tracking analysis.
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Tecno Chem CO LTD microcomputed tomography scanxmate-e090
The extraction and identification of BMSCs. (A) Ordinary optical microscope observations of BMSC morphology (×100). (B) Ordinary optical microscope analyses of adipogenic and osteogenic differentiation in BMSCs in vitro (×200). (C) Flow cytometer measurement of surface antigens (positive: CD29, CD90, and CD44; negative: CD45 and CD34). (D) Transmission electron microscope observations of BMSC‐Exos. (E) NTA analyses of BMSC‐Exo size. (F) Western blot examination of the expression of Exo surface marker proteins Alix, <t>CD63,</t> <t>GM130,</t> cytochrome c , and <t>calnexin.</t> (G) Laser scanning microscope evaluation of Exo uptake in H9C2 cells. BMSC, bone marrow stromal cells; Exo, exosome; NTA, nanoparticle tracking analysis.
Microcomputed Tomography Scanxmate E090, supplied by Tecno Chem CO LTD, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Siemens AG mri scanner siemens magnetom verio 3.0t
The extraction and identification of BMSCs. (A) Ordinary optical microscope observations of BMSC morphology (×100). (B) Ordinary optical microscope analyses of adipogenic and osteogenic differentiation in BMSCs in vitro (×200). (C) Flow cytometer measurement of surface antigens (positive: CD29, CD90, and CD44; negative: CD45 and CD34). (D) Transmission electron microscope observations of BMSC‐Exos. (E) NTA analyses of BMSC‐Exo size. (F) Western blot examination of the expression of Exo surface marker proteins Alix, <t>CD63,</t> <t>GM130,</t> cytochrome c , and <t>calnexin.</t> (G) Laser scanning microscope evaluation of Exo uptake in H9C2 cells. BMSC, bone marrow stromal cells; Exo, exosome; NTA, nanoparticle tracking analysis.
Mri Scanner Siemens Magnetom Verio 3.0t, supplied by Siemens AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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PEQLAB nanodrop 1000 nd-1000
The extraction and identification of BMSCs. (A) Ordinary optical microscope observations of BMSC morphology (×100). (B) Ordinary optical microscope analyses of adipogenic and osteogenic differentiation in BMSCs in vitro (×200). (C) Flow cytometer measurement of surface antigens (positive: CD29, CD90, and CD44; negative: CD45 and CD34). (D) Transmission electron microscope observations of BMSC‐Exos. (E) NTA analyses of BMSC‐Exo size. (F) Western blot examination of the expression of Exo surface marker proteins Alix, <t>CD63,</t> <t>GM130,</t> cytochrome c , and <t>calnexin.</t> (G) Laser scanning microscope evaluation of Exo uptake in H9C2 cells. BMSC, bone marrow stromal cells; Exo, exosome; NTA, nanoparticle tracking analysis.
Nanodrop 1000 Nd 1000, supplied by PEQLAB, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Hamamatsu ccd-camera c-8800-21
The extraction and identification of BMSCs. (A) Ordinary optical microscope observations of BMSC morphology (×100). (B) Ordinary optical microscope analyses of adipogenic and osteogenic differentiation in BMSCs in vitro (×200). (C) Flow cytometer measurement of surface antigens (positive: CD29, CD90, and CD44; negative: CD45 and CD34). (D) Transmission electron microscope observations of BMSC‐Exos. (E) NTA analyses of BMSC‐Exo size. (F) Western blot examination of the expression of Exo surface marker proteins Alix, <t>CD63,</t> <t>GM130,</t> cytochrome c , and <t>calnexin.</t> (G) Laser scanning microscope evaluation of Exo uptake in H9C2 cells. BMSC, bone marrow stromal cells; Exo, exosome; NTA, nanoparticle tracking analysis.
Ccd Camera C 8800 21, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Larodan tricaprin
The extraction and identification of BMSCs. (A) Ordinary optical microscope observations of BMSC morphology (×100). (B) Ordinary optical microscope analyses of adipogenic and osteogenic differentiation in BMSCs in vitro (×200). (C) Flow cytometer measurement of surface antigens (positive: CD29, CD90, and CD44; negative: CD45 and CD34). (D) Transmission electron microscope observations of BMSC‐Exos. (E) NTA analyses of BMSC‐Exo size. (F) Western blot examination of the expression of Exo surface marker proteins Alix, <t>CD63,</t> <t>GM130,</t> cytochrome c , and <t>calnexin.</t> (G) Laser scanning microscope evaluation of Exo uptake in H9C2 cells. BMSC, bone marrow stromal cells; Exo, exosome; NTA, nanoparticle tracking analysis.
Tricaprin, supplied by Larodan, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The extraction and identification of BMSCs. (A) Ordinary optical microscope observations of BMSC morphology (×100). (B) Ordinary optical microscope analyses of adipogenic and osteogenic differentiation in BMSCs in vitro (×200). (C) Flow cytometer measurement of surface antigens (positive: CD29, CD90, and CD44; negative: CD45 and CD34). (D) Transmission electron microscope observations of BMSC‐Exos. (E) NTA analyses of BMSC‐Exo size. (F) Western blot examination of the expression of Exo surface marker proteins Alix, CD63, GM130, cytochrome c , and calnexin. (G) Laser scanning microscope evaluation of Exo uptake in H9C2 cells. BMSC, bone marrow stromal cells; Exo, exosome; NTA, nanoparticle tracking analysis.

Journal: Immunity, Inflammation and Disease

Article Title: Bone marrow stromal cell‐derived exosomes improve oxidative stress and pyroptosis in doxorubicin‐induced myocardial injury in vitro by regulating the transcription of GSDMD through the PI3K‐AKT‐Foxo1 pathway

doi: 10.1002/iid3.810

Figure Lengend Snippet: The extraction and identification of BMSCs. (A) Ordinary optical microscope observations of BMSC morphology (×100). (B) Ordinary optical microscope analyses of adipogenic and osteogenic differentiation in BMSCs in vitro (×200). (C) Flow cytometer measurement of surface antigens (positive: CD29, CD90, and CD44; negative: CD45 and CD34). (D) Transmission electron microscope observations of BMSC‐Exos. (E) NTA analyses of BMSC‐Exo size. (F) Western blot examination of the expression of Exo surface marker proteins Alix, CD63, GM130, cytochrome c , and calnexin. (G) Laser scanning microscope evaluation of Exo uptake in H9C2 cells. BMSC, bone marrow stromal cells; Exo, exosome; NTA, nanoparticle tracking analysis.

Article Snippet: The membranes were supplemented respectively with diluted primary antibodies against Alix (ab275377, 1:1000; Abcam), CD63 (PA5‐92370, 1:1000; Thermo Fisher Scientific), GM130 (PA5‐95727, 1:2000; Thermo Fisher Scientific), cytochrome c (ab133504, 1:1000; Abcam), calnexin (ab133615, 1:1000; Abcam), nucleotide‐binding oligomerization domain, leucine rich repeat and pyrin domain containing 3 (NLRP3, ab263899, 1:1200; Abcam), apoptosis‐associated speck‐like protein containing A CARD (ASC, 307560, 1:1000; Abcam), cleaved caspase‐1 (89332S, 1:1000; Cell Signaling Technologies [CST]), interleukin (IL)‐18 (67775S, 1:1000; CST), cleaved IL‐1β (63124S, 1:1000; CST), active N‐terminal fragment of GSDMS (NT‐GSDMD, 10137S, 1:1000; CST), phosphorylated‐phosphatidylinositol 3‐kinase (p‐PI3K, ab278545, 1:1000; Abcam), p‐protein kinase B (p‐AKT, 4060T, 1:1000; CST), total‐AKT (4685S, 1:1000; CST), p‐mechanistic target of rapamycin kinase (p‐mTOR, ab109268, 1:1000; Abcam), p‐Foxo1 (ab259337, 1:1000; Abcam), acetyl‐Foxo1 (PA5‐104560, 1:1000; Thermo Fisher Scientific), and β‐actin (ab8226, 1:2500; Abcam) for overnight probing at 4°C.

Techniques: Microscopy, In Vitro, Flow Cytometry, Transmission Assay, Western Blot, Expressing, Marker, Laser-Scanning Microscopy